Table 1.
Primer | Sequence* |
---|---|
6MIEpF | 5'-tct ctc gag agt taa aga tca gcg ggt ac-3' |
6MIEpF-732 | 5'-agt cgg tac cgg cga atg aga act cta aaa gct c-3' |
6MIEpF-552 | 5'-agt cgg tac cta ctg tgg ttg ggg tct ttc cta c-3' |
6MIEpF-531 | 5'-acc ggt acc tac cca ggc taa cga gaa cc-3' |
6MIEpF-381 | 5'-agt cgg tac cac att cct gtt tca tga tgt gta gc-3' |
6MIEpF-214 | 5'-agt cgg tac ctc ctg ttt ttg agt aag ata tga c-3' |
6MIEpF-165 | 5'-agt cgg tac cag cta att tcc att cca tat ttg tc-3' |
6MIEpF-102 | 5'-agt cgg tac cta cag cga ttg gct cct tca tcc tc-3' |
6MIEpR | 5'-agt cct cga gca ctg aac tgg ctg taa ctt ctg c-3' |
6MIEpex2R | 5'-tct aag ctt cag caa tcc aat aat tga tg-3' |
6MIEpex3R | 5'-cat aag ctt gca tac gtt cct cat tgg at-3' |
6MIEpex4R | 5'-cat aag ctt cca aag ttt tga att ctt ca-3' |
6MIEpex5R | 5'-cat aag ctt ttt gga tgc aag tgc caa cg-3' |
DsRed2-HindF | 5'-acc aag ctt tac cgg tcg cca cca tgg cct-3' |
DsRed2-HindR | 5'-acc aag ctt tta tct aga tcc ggt gga tcc-3' |
ORF12TnFw | 5'-tat ctc gag agg tac cgg tga ctt cag ag-3' |
ORF12TnRv | 5'-cga gga tcc aat caa cca atc aga cct-3' |
ORF13TnFw | 5'-gag gat ccg tac cca caa tat caa gtg gt-3' |
ORF13TnRv | 5'-gac tcg agc cta ttc gtg tca tct aga tgg-3' |
*:underlines indicate restriction enzyme sites.