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. 2011 Jan 21;6(1):e14571. doi: 10.1371/journal.pone.0014571

Figure 3. T. cruzi-infected and HKTC-immunized mice develop DTH specific for a number of cardiac and non-cardiac proteins.

Figure 3

A/J mice were infected with T. cruzi, immunized with HKTC, immunized with HKLA, or injected with PBS. (A) At 21 d.p.i., antigen-specific DTH responses to a panel of cardiac and non-cardiac antigens were measured by a standard 24-hour ear swelling assay. (B) DTH responses specific for HKTC and myosin were measured in A/J mice immunized heat-killed T. cruzi epimatigotes (HKTC), T. cruzi tissue-culture trypomastigotes (HKTCtct) T. cruzi cultured metacyclic trypomastigotes (HKTCcmt), supernatant from T. cruzi-infected myoblast cultures (supernatant), or injected with PBS. Data represent the net ear swelling response over the basal response to an irrelevant antigen (BSA). Error bars indicate SEM (n = 4). Data are representative of two independent experiments. * P<0.05 compared to the PBS control group.