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. 2011 Jan 21;6(1):e14571. doi: 10.1371/journal.pone.0014571

Figure 5. T. cruzi-infection but not HKTC immunization induces robust autoreactive Th1 and Th17 responses.

Figure 5

A/J mice were infected with T. cruzi (INF), or immunized with HKTC (HKTC), or PBS (PBS). At 21 d.p.i., splenocytes were isolated and stimulated with HKTC or Myo 4. Intracellular cytokine staining of CD90+CD4+ splenocytes stimulated with anti-CD3 for IFN-γ, IL-4, and IL-17 was analyzed via flow cytometry. (A) The mean percentage of cytokine-positive cells is shown. Error bars indicate SEM (n = 4 for INF and HKTC; n = 3 for PBS). Data are representative of two independent experiments. (B) Representative plots of the cells quantified in panel A are shown. The mean percentage and SEM of IFN-γ+IL-17-, IFN-γ+IL-17+, and IFN-γ−IL-17+ cells are indicated.