Skip to main content
. 2011 Jan 21;6(1):e16360. doi: 10.1371/journal.pone.0016360

Figure 1. A Southern blot hybridization of genomic DNA of tobacco plants hybridized with GST probe.

Figure 1

C- non-transgenic plant, P-ECoRI- HindIII digested 1 Kb fragment of GST-pCAMBIA 1301 plasmid as positive control,1–6 transgenic plants showing the bands of different sizes, indicating the integration of GST gene at different loci in the genome of transgenic plants. B RT-PCR of PCR positive transgenic tobacco plants. 1-pCAMBIA 1301-GST plasmid, 2-non-transgenic plant, 3–12 PCR positive transgenic plant samples. All PCR positive transgenic lines were found positive in RT-PCR. Actin gene was used as the house- keeping gene.