Table 1.
Antibodies
| Antibody | References | Source | Applicationa | ||
|---|---|---|---|---|---|
| IF | IB | IP | |||
| Goat anti-WNT2 | 27 | BioVision | 1: 200 | ||
| Goat anti-WNT2 | 16, 28 | Santa Cruz | 1: 75 | 1: 200 | 4.0 μ |
| Rabbit anti-GSK-3β | 30, 31 | Santa Cruz | 1: 100 | 1: 300 | |
| Rabbit anti-β-catenin | 32, 33 | Chemicon | 1:500 | 1: 1,000 | |
| Mouse anti-PCNA | 34 | Sigma | 1: 1,500 | ||
| Rabbit anti-cyclin D2 | 29 | Santa Cruz | 1: 500 | ||
| Alexa Fluor® 488 goat anti-rabbit | Molecular Probes | 1:500 | |||
| Alexa Fluor® 488 goat anti-mouse | Molecular Probes | 1:500 | |||
| Alexa Fluor® 488 rabbit anti-goat | Molecular Probes | 1:500 | |||
| Alexa Fluor® 594 goat anti-rabbit | Molecular Probes | 1:300 | |||
| Alexa Fluor® 594 goat anti-mouse | Molecular Probes | 1:300 | |||
| Alexa Fluor® 680 goat anti-rabbit | Molecular Probes | 1: 10,000 | |||
| Alexa Fluor® 680 donkey-anti goat | Molecular Probes | 1: 10,000 | |||
| Alexa Fluor® 680 goat anti-mouse | Molecular Probes | 1: 10,000 | |||
a
IF, immunofluorescence; IB, immunoblotting; IP, immunoprecipitation