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. 2011 Jan 21;6(1):e16306. doi: 10.1371/journal.pone.0016306

Figure 1. Dose dependent downregulation of secreted MMPs by curcumin on cag+ve and cag-ve Hp-infected AGS cells.

Figure 1

AGS cells cocultured with SS1 (cag+ve) and AM1 (cag-ve) Hp strains were pretreated with 20 µM, 40 µM, 60 µM doses of curcumin and the effect of increasing doses of curcumin on Hp viability during coculture of Hp and AGS cells for 24 h were represented in the histogram (A). The MMP-3 and -9 levels in the conditioned media after 24 h were analyzed. Western blots (B) were performed using equal volumes of concentrated media followed by probing with polyclonal anti-MMP-3, and -GAPDH and monoclonal anti-MMP-9 antibodies. AGS cells alone were considered as control. AGS cells cocultured with SS1 Hp strain were pretreated with curcumin or TT or only-antibiotics or omeprazole and the MMP levels in the conditioned media after 24 h were assessed. Histographic representation of Hp-viability (C) after 24 h of coculture against various treatments. Concentrated conditioned media from each group of treatment were analyzed by Western blot (D) for MMP-3 and MMP-9 secretion level by probing with polyclonal anti-MMP-3, and GAPDH and, monoclonal anti-MMP-9 antibodies. Fold changes of protein were calculated from the above blots and two other representative blots from independent experiments.