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. 2011 Jan 21;6(1):e16306. doi: 10.1371/journal.pone.0016306

Figure 5. Curcumin regulates MMP-3 and -9 activity and expression in both cag+ve and cag−ve Hp-infected mice.

Figure 5

Two weeks Hp (either SS1 or AM1) infected C57BL/6 mice were orally-fed with curcumin for 7-days. The activities of MMP-3, and MMP-9, -2 in the PBS extracts of respective mouse gastric tissues were analyzed by casein (A) and gelatin (B) zymograms respectively. Histographic representations of caseinolytic (C) and gelatinolytic (D) activities from the above zymograms and three other representative zymograms from independent experiments. Error bars = ±SEM. *, p<0.001; ns, nonsignificant vs. appropriate control. Western blot analysis of MMP-3, -9 and -2 proteins from respective tissues, GAPDH served as loading-control (E). RT-PCR analysis of MMP-3, -9 and -2 mRNA expressions in respective tissues while GAPDH served as control (F).