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. Author manuscript; available in PMC: 2011 Jan 21.
Published in final edited form as: Methods Mol Biol. 2010;627:75–90. doi: 10.1007/978-1-60761-670-2_4

Figure 5. Loading Streptavidin (SA) Sensor Chip.

Figure 5

A typical sensorgram generated during the loading of biotinylated protein to flow cells 1 and 2. The flow cell surface was stabilized with 3 × 20 μl injections of Regeneration Buffer before loading of biotinylated-Gαi1 (260 nM final concentration, as diluted in Biotin-conjugate Immobilization Buffer), one flow cell at a time, until saturation is observed (note plateau in sensorgram curves).