Command	Explanation
DEFINE APROG galpha	//a unique name for this block
PARAM %position	//defines ‘position’ is the only variable
FLOW 20	//sets the flow rate (units μl/min)
FLOWPATH 1,2,3,4	//can be modified to inject only two flow cells
KINJECT %position 200 300	//performs a stabilized injection, 200 μl volume and 300 sec dissociation time
END
DEFINE APROG loadGST	//specifies what to load to sensor chip surface
FLOW 5
FLOWPATH 1,2	//specifies flow cells to load
INJECT R2F3 15	//specifies position to retrieve GST from stock tube and amount (in μl)
EXTRACLEAN
END
DEFINE APROG loadGSTfusion	//specifies what to load to sensor chip
FLOW 5
FLOWPATH 3,4
INJECT R2F4 20	//specifies position to retrieve GST-fusion protein and amount (in μl)
EXTRACLEAN
END
DEFINE APROG glycine	//specifies removal of GST/GST-fusion from antibody surface
FLOW 20
FLOWPATH 1,2,3,4
INJECT R2F6 40
EXTRACLEAN
END
MAIN
RACK 1 THERMO_C	//specifies which thermal block is to be found in the rack positions
RACK 2 THERMO_A
RACK R Reag_A
DETECTION 1,2,3,4
APROG loadGST	//loads a GST control
APROG loadGSTfusion	//loads the experiment
APROG galpha R2A1	//injection of 200 μl of the sample in rack position R2A1
APROG glycine	//regenerates the binding surface
APPEND Standby	//puts the machine in standby mode
END