Figure 5. The effects of plasma on cells are dependent on ROS concentration.

(A) Cells on cover slips overlaid with 100 µl cell culture media were treated with plasma (1.55 J/cm²), followed by dilution in 2 ml of media at the indicated holding time after treatment. Cell lysates were collected 1 hour after dilution in 2 ml of media. Immunoblots with γ-H2AX or α-tubulin are shown. (B) Medium was separately treated with plasma and then diluted immediately after treatment as indicated. Cells were then exposed to the treated and diluted medium for 1 min, followed by 1-hour incubation in 2 ml of fresh medium. Immunoblots with γ-H2AX or α-tubulin are shown.