Figure 6. Effects of DBD Plasma are mediated by neutral species generated in the medium.

(A) Cells were subjected to direct treatment with plasma (D) or to medium (100 µl) that was exposed to plasma and then transferred to the cells (separate, S). (A, B) Representative immunoblots with γ-H2AX (top) or α-tubulin (bottom) are shown. The graphs below the immunoblots show quantification using Odyssey. The γ-H2AX signal was normalized to the amount of α-tubulin. Data are expressed relative to lowest dose, which was set at 1.0. (B) Medium (100 µl separated treatment) was subjected to plasma and transferred to cells after holding for 1 to 60 min. After 1-minute incubation with cells, cover slips with treated medium and cells were transferred to a dish with 2 ml of medium.