Figure 9. Plasma treatment does not induce the formation of thymine dimers.

MCF10A cells grown on glass coverslips were incubated with (A) or without (B) 4 mM NAC for 2 h, followed by treatment with direct plasma (2.3 J/cm²), UV (10 J/m²), H₂O₂ (200 µM), or a no treatment (NT) control. Cells were allowed to recover for 1 h, then fixed and immunostained with TDM-2 primary antibody (kindly provided by Dr. Toshio Mori at the Nara Medical University, Kashihara, Nara, Japan) and Alexa Fluor 594 anti-mouse secondary antibody to detect cyclobutane pyrimidine dimers.