Figure 1. Generation of transgenic mice.

(A) Diagram of the transgene constructs. The transgenes contain the gamma-sarcoglycan (GSG) promoter fused with the EGFP coding sequence and followed by SV40 polyadenylation signals (poly A). Insertion of 23 or 200 CAG repeats was made downstream of the EGFP stop codon and before the poly A sequence. Locations of primers (f1, f2, r1 and r2) used for PCR are marked. (B) PCR-based Southern blot analysis. Tail DNA from different founder animals (as indicated by the numbers) generated from the three constructs were PCR-amplified using primers f1 and r2, which generated fragments of 632 bp, 701 bp and 1,232 bp, from CAG₀, CAG₂₃ and CAG₂₀₀ transgenes, respectively. Upper panels, ethidium bromide-stained agarose gels; lower panels, blots of PCR products hybridized with a CAG₁₀ probe.