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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: Anal Chem. 2010 Oct 15;82(20):8476–8484. doi: 10.1021/ac101079v

Table 3.

Correlation of ion abundances from MALDI IMS ionsa of PC molecular species across regions of rat brain relative to ion abundances of PC molecular species obtained by LC MS/MS and LC/MS analysis of dissected brain regions. Statistical analysis employed Pearson product moment analysis.

Ion Molecular species Person product moments
LC/MS/MSb Abundances LC/MS/MSc Relative Molar Abundance LC/MSd Relative Molar Abundance
m/z 734.6 16:0/16:0 PC 0.88 0.90 0.92
m/z 760.6 16:0/18:1 PC 0.88 - -
m/z 788.6 18:0/18:1 PC 0.27 0.96 0.86
m/z 834.6 18:0/22:6 PC 0.88 0.96 0.76
a

MALDI ion abundances normalized by abundance of 16:0/18:1 PC averaged over entire brain region.

b

Ion intensities of each molecular species obtained by targeted LC/MS/MS (SRM) were normalized to the signal for internal standard ([2H6]-16:0/16:0 PC) and microdissected tissue wet weight. Averaged over four separate samples.

c

Ion intensities of each molecular species obtained by targeted LC/MS/MS (SRM) were normalized to the signal for 16:0/18:1 PC in the microdissected tissue. Averaged over four separate samples.

d

Ion intensities of each molecular species obtained by LC/MS (full mass scanning) were normalized to the signal for 16:0/18:1 PC in the microdissected tissue. Averaged over four separate samples.