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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Hepatology. 2010 Oct 21;53(1):116–126. doi: 10.1002/hep.24006

Figure 2. Hepatic ATGL knockdown induces steatosis.

Figure 2

(A) Liver weights in mice treated with control or ATGL shRNA in both chow and HF diet groups (n = 8-10). (B) Liver sections from these mice were stained with hematoxylin and eosin and imaged at 20× magnification. (C) Liver TAG was quantified from mice fed chow or high fat diets (n = 8-10). (D) In order to determine composition of TAG, lipids extracted from livers of chow-fed mice were separated by TLC and TAG was methylated with 5% HCl in methanol to produce fatty acid methyl esters (FAMEs), which were analyzed by GC (n = 6). Data are presented as means ± SEM. *P<0.05 vs control shRNA group.