Pretreatment with CX3CL1 (10 nM) blocks morphine’s neurophysiological effects (hyperpolarization and reduction of input resistance) in 8/14 PAG neurons (A, B) but not in the other 6/14 PAG neurons (C, D), resulting in a blunting of morphine’s effect when data from all cells are combined (Figure 6E, F).
* indicates significant difference from the morphine group and # indicates significant difference from the MOR + CX3CL1 group by post-hoc Dunn’s tests (p < 0.05). Group data are presented as mean ± SEM.