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Effect of GSPE on H2O2-induced generation of reactive oxygen species in HLEB-3 cells. HLEB-3 cells were pretreated with 20 mg/l GSPE for 12 h followed by treatment with 100 μmol/l H2O2. The ROS production was measured by DCF flow cytometry as described in Methods. The result showed GSPE pretreatment reduced ROS generation induced by H2O2.
