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Effect of GSPE on H2O2-induced phosphorylation of the MAPK pathway. HLEB-3 cells were incubated with 20 mg/l GSPE for 12 h. Cells were harvested at 1, 2, and 3 h time points after treated with 100 μmol/l H2O2, and p38 and JNK activities were evaluated by western blotting. GSPE markedly reduced H2O2induced phosphorylation of p38 and JNK at each time point studied. The total amount of JNK and p38 remained unchanged at each time point studied.
