Table 1. Primers for KIF21A.
| Exon | 5′ to 3′ primer sequence | PCR conditions |
|---|---|---|
| Kfi21a_x1Fn |
ctgttggcttctccacagg |
52 °C/35 cycles |
| Kfi21a_x1Rn |
gggactcactgcctcagttt |
|
| Kfi21a_x2Fn |
tcatgattttgggggattgt |
53 °C/35 cycles |
| Kfi21a_x2Rn |
caaaaatgaaagcgcaactg |
|
| Kif21a_x3F |
tcagttgcgctttcatttttg |
53 °C/35 cycles |
| Kif21a_x3R |
ctccaacctgggtgacagaa |
|
| Kif21a _x4F |
tagcctcattcattttaatgtgtt |
59 °C/35 cycles |
| Kif21a _x4R |
gatcttaattccatgtcatgcttc |
|
| Kif21a _x5F |
tgcctgtaactgaactaataatgtga |
59 °C/35 cycles |
| Kif21a _x5R |
atggctgaccagcttcaact |
|
| Kif21a _x6F |
tttggctttatgcctgtttc |
59 °C/35 cycles |
| Kif21a _x6R |
tgaggagattggagattcagtg |
|
| Kif21a_x7F |
cttatttctgtttcaaagaattagta |
59 °C/35 cycles |
| Kif21a_x7R |
cctacacctcaagggatgct |
|
| Kif21a _x8F |
caggggcttttaaatttgct |
59 °C/35 cycles |
| Kif21a _x8R |
ctccaaaaggaaggaggaca |
|
| Kif21a_x9Fn |
tggtcttgaactcctgacctc |
59 °C/ 35 cycles |
| Kif21a_x9rn |
tgccctccagaagttaatcc |
|
| Kif21a_x10F |
tgtggtctgctcatgtaataaagg |
53 °C/35 cycles |
| Kif21a_x10R |
ggaatatgacatcaagggaaagg |
|
| Kif21a_x11Fn |
ccacagagaaaaatgctcccta |
59 °C/35 cycles |
| Kif21a_x11Rn |
tgaatggaatgcaaaagcag |
|
| Kif21a _x12F |
gcatccaagcatgcctaatc |
59 °C/35 cycles |
| Kif21a _x13R |
tttaggagcagcccagctta |
|
| Kif21a_x13Fn |
tgattggcaatttccattttt |
59 °C/35 cycles |
| Kif21a_x13Rn |
gactccccaacacaatgctt |
|
| Kif21a _x14F |
gttggggagtcaggggtaga |
56 °C/35 cycles |
| Kif21a _x14R |
taaagccttggaaggcaaatg |
|
| Kif21a _x15F |
cattcaccttttggttgttgg |
59 °C/35 cycles |
| Kif21a _x15R |
aggcacaaactttgacttgc |
|
| Kif21a _x16F |
gacaccctagtcttctgagatgtg |
59 °C/35 cycles |
| Kif21a _x16R |
ttgccaaaggaaattacatca |
|
| Kif21a _x17F |
taaacgtgcagcaaaactgc |
59 °C/35 cycles |
| Kif21a _x17R |
tgcttatctattgtccttaacctgc |
|
| Kif21a x18F |
tggccgttaatactgaatgttg |
56 °C/35cycles |
| Kif21a x18R |
aaagcaggttggattttaagaaa |
|
| Kif21a_x19F |
ccatttggaagaaaccttctg |
56 °C/35 cycles |
| Kif21a_x19R |
tgcactgccaaataatgagc |
|
| Kif21a _x20–21F |
ggcaacaaatggaaacaggt |
59 °C/35 cycles |
| Kif21a _x20–21R |
tggcatacatgtaaaacctaagc |
|
| Kif21a _x22F |
ccctatgtttcttggggtaatgat |
59 °C/35 cycles |
| Kif21a _x22R |
tccttattacaaagcaaagggtta |
|
| Kif21a _x23–24F |
ttactggaggagctgggatg |
59 °C/35 cycles |
| Kif21a _x23–24R |
tagtgtgtttgtgggcatgg |
|
| Kif21a _x25_ 26F |
actaaaaccatcgtgcccat |
59 °C/35 cycles |
| Kif21a _x25_ 26R |
gctttagtaaaaccatgccctc |
|
| Kif21a 26F |
tggcctagtgaatagcacttagaa |
59 °C/35 cycles |
| Kif21a 26R |
cagttaccacttaaagggaaatatga |
|
| Kif21a _x27F |
cacacctaggaaaagacacgct |
56 °C/35 cycles |
| Kif21a _x27R |
ggggagacaacacctagcaa |
|
| Kif21a_x28F |
caagtaataatctttctgaggttcca |
56 °C/35 cycles |
| Kif21a_x28R |
accacagcaccagcctaaat |
|
| Kif21a _x29F |
ttgttcagaatgcattttatcttaca |
59 °C/35 cycles |
| Kif21a _x29R |
gcatggttcctttcccatt |
|
| Kif21a _x30F |
agcagggcactatgaaggaa |
56 °C/35 cycles |
| Kif21a _x30R |
tttatctaaaaggtatgaccacaaaa |
|
| Kif21a_x31Fn |
tgtctcattccctttcacca |
56 °C/35 cycles |
| Kif21a_x31Rn |
caacagacttgatctgaaggaga |
|
| Kif21a _x32F |
gcttaaaagagagcagtfctgga |
59 °C/35 cycles |
| Kif21a _x32R |
ggttgaaccagattatccga |
|
| Kif21a _x33F |
tgaagttaggatccttgtggtatg |
59 °C/35 cycles |
| Kif21a _x33R |
tgggaagtggacaggtatacaa |
|
| Kif21a _x34F |
tgtgttaggtgctgtgctagg |
56 °C/35 cycles |
| Kif21a _x34R |
aaggacacaagagacatttagagg |
|
| Kif21a _x35F |
gcccaagatcccatctctaa |
56 °C/35 cycles |
| Kif21a _x35R |
ccactaactatgaatgaaggaaaaga |
|
| Kif21a_x36Fn |
ctccagcctgggaaacatag |
59 °C/35 cycles |
| Kif21a_x36Rn |
ggcctgattaatattatctgtaaatga |
|
| Kif21a _x37F |
ctttctccagccaattccaa |
59 °C/35cycles |
| Kif21a _x37R |
aacctggggtgcctaaattc |
|
| Kif21a _x38F |
tgtaaagggcacatggtaacaa |
59 °C/35 cycles |
| Kif21a _x38R | gcagttgaattcagatatattttcca |