Table 3. List of primers for TUBB3.
| Exon | 5’ to 3’ sequence | Product size | Temperature/cycles |
|---|---|---|---|
| TUBB3X1F |
ggccgcggctataagag |
272 |
56 °C /35 |
| TUBB3X1R |
catccctttgttgcaggttc |
|
|
| TUBB3X2F |
tgggtcaaaagccctaatttt |
317 |
56 °C /35 |
| TUBB3X2R |
ctgagagctggtgagtccag |
|
|
| TUBB3X3F |
gctcttaggatgtgagcagga |
323 |
56 °C /35 |
| TUBB3X3R |
ggagctgaccattccttgtt |
|
|
| TUBB3X4-1F |
atgagaaggggtgctcagtg |
489 |
56 °C /35 |
| TUBB3X4-1R |
ctcgttgtcgatgcagtagg |
|
|
| TUBB3X4-2F |
cgcatcatgaacaccttcag |
498 |
56 °C/35 |
| TUBB3X4-2R |
gtccacctccttcatggaca |
|
|
| TUBB3X4-3F |
agctcacccagcagatgttc |
594 |
56 °C /35 |
| TUBB3X4-3R | gaggggaaagcagggtgt |
Sequences in Table are based on [22].