Table 1. Antibodies used for western blotting.
| Antibody | Dilution | Clone/catalog | Gel conditions |
|---|---|---|---|
| Collagen I |
1:5,000 |
Anti-CN1 |
LB |
| Collagen IV |
1:1,000 |
IV-4H122 |
NRB |
| Laminin α3 |
1:1,000 |
BM1656 |
LB |
| Laminin α5 |
1:1,000 |
sc-201454 |
LB |
| Laminin β3 |
1:1,000 |
sc-55834 |
LB |
| Laminin γ1 |
1:500 |
Clone 175 |
LB |
| Laminin γ2 |
1:1,000 |
2E83 |
LB |
| Integrin α1 |
1:500 |
FB123 |
NRB |
| Integrin α2 |
1:1,000 |
AB19363 |
LB |
| Integrin α3/α6 | 1:10,000 | AA6A7 | NRB |
1Immunohistochemical research (Gilbertsville, PA). 2Calbiochem (San Diego, CA). 3Millipore (Billerica, MA). 4Santa Cruz Biotechnologies (Santa Cruz, CA). 5BD Biosciences (Franklin Lakes, NJ). 6BM165; Dr. Robert Burgeson, Harvard University, Cambridge, MA and Dr. Peter Marinkovich, Stanford University, Palo Alto, CA, 7Clone 4C7; Millipore, Billerica, MA. In the table, NRB indicates non-reducing buffer and LB indicates Laemmli buffer.