Table 2.

DNA strands of cloned protospacers showing the sequence corresponding to crRNAs.

Construct	Locus/spacer	Sequence	Mismatches	Positions
(A) S. solfataricus P2
ATV618	D/29	CCATTTTGATAACTAGATGTGGAACCGAAGTTTACTACTAGTT	0
	D/29	CCATTTTGATAACTAGATGTGGAACCAAAGTTTACTACTAGTT	1	24
	D/29	CCATTTTGATAACTAGATGTAGAACCAAAGTTTACTACTAGTT	2	18, 24
	D/29	CCATTTTGATAACTAGATGTAGAGCCAAAGTTTACTACTAGTT	3	18, 21, 24
ATV892	A/28	TCCCTCGCTAACGTTCAAATCTTTCAATAATTTTTGCACGT	0
	A/28	TCCCTCGCTAACATTCAAATCCTTCAATAATTTTTGCACGT	2	10, 19
ATV145	D/28	CCGAAAAGCCAATCCCAAGATACATCATCGCAGAAATATTCA	0
Protospacer	A/28	TCCCTCGCTAACGTTCAAATCTTTCAATAATTTTTGCACGT	0
	A/28	TCCTTCGCTAACGTTCAAATCTTTCAATAATTTTTGCACGT	1	1
	A/28	TCCCTCGCTAACGTTCAAATCCTTCAATAATTTTTGCACGT	1	19
	A/28	TCCCTCGCTAACGTTCAAATCTTTCAATAATTTTTGCACGC	1	38
	A/28	TCCTTCGCTAACGTTCAAATCTTTCAATAATTTTTGCACGC	2	1, 38
	A/28	TCCCTCGCTAACGTTCAAATCTTTCAATAATTTTTGCGGCC	4	35–38
	A/28	TCCCTCGCTAACGTTCAAATCTTTCAATAATCGGCCGCACA	10	29–38
(B) S. islandicus REY15A
Protospacer	2/45	CCATTAGGAGTCGTAGCACAGGGAGCTGTACAGTCACAGAA	0
	2/45	CCACTAGGAGTCGTAGCACAGGGAGCTGTACAGTCACAGAA	1	1
	2/45	CCATTAGGAGTCGTAGCACAGAGAGCTGTACAGTCACAGAA	1	19
	2/45	CCATTAGGAGTCGTAGCACAGGGAGCTGTACAGTCACAGAG	1	38
	2/45	CCATTAGGAGTCGTAGCACAGGGAGCTGTACAGTCAGTCGA	4	34–37
	2/45	CCATTAGGAGTCGTAGCACAGGGAGCTGTACGTCGACCTGC	8	29–32, 35–38

PAM motifs are in bold type and underlined at the 5′ ends and the positions and identities of mismatches that were introduced are also in bold type and underlined for: (A) genes encoding ATV618, ATV892 and ATV145 and cloned protospacers of S. solfataricus P2 CRISPR loci, and (B) cloned protospacers of S. islandicus REY15A in CRISPR locus 2.