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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Immunol. 2010 Oct 22;185(11):6535–6544. doi: 10.4049/jimmunol.1002009

FIGURE 3.

FIGURE 3

Cox-2 siRNA attenuates TLR ligand-induced β-defensins. NHEKs were transfected with 3 μM Cox-2 siRNA at 0 h and then again at 24 h. Twenty-fours after the last tranfection cells were harvested for Western blotting or treated with AMP-inducing stimuli for 24 h. A, Transfection of Cox siRNA constructs knocked-down Cox-2 protein by >99%. B, Poly(I:C) significantly induced hBD2 mRNA ~30-fold (#p < 0.05). Cox-2 siRNA significantly attenuated poly(I:C)-induced hBD2 mRNA (*p < 0.05). C, Poly(I:C) significanlty induced hBD3 mRNA ~20-fold (#p < 0.05). Cox-2 siRNA significantly attenuated poly(I:C)-induced hBD3 mRNA (*p < 0.05). D, UVB induced hBD2 mRNA ~6-fold (#p < 0.05). Cox-2 siRNA significantly attenuated UVB-induced hBD2 mRNA (*p < 0.05). E, UVB induced hBD3 mRNA ~3-fold (#p < 0.05). Cox-2 siRNA significantly attenuated UVB-induced hBD3 mRNA (*p < 0.05).