FIGURE 7.
Cox-2 enhances S. aureus killing. NHEK lysates were incubated with 106 bacteria at an MOI of 20 for 1, 3, or 5 h. Bacteria was quantified after 24 h. NHEKs pretreated with SC-58125 (10 μM) and then treated with poly(I:C) (10 μg/ml) showed a reduced ability to kill S. aureus strains (A) ΔmprF and (B) Sa113 when compared with NHEKs treated with poly(I:C) in the absence of SC-58125. NHEK lysates were incubated with 107 bacteria at an MOI of 20 for 0.5, 1, or 3 h. Bacteria was quantified after 24 h. NHEKs treated with 15d-PGJ2 (500 nM) showed a reduced ability to kill S. aureus strains (C) ΔmprF and (D) Sa113 when compared with vehicle-treated NHEKs. Results are representative of three separate experiments.