FIG. 5.

Immunofluorescence detection of transgenic hPTH in parotid glands. Parotid glands were removed 2 days after transduction, embedded in O.C.T. compound, and frozen in liquid nitrogen; 15-μm sections were fixed in 4% paraformaldehyde. Sections were immunostained for hPTH (green) and nuclei were stained with DAPI (blue). (A) Saline-treated glands. (B) Ad.hPTH-treated glands. In each panel a phase-contrast image is shown on the left for visualization of gland morphology. Next, an immunostained image is shown. In the phase-contrast images are two boxes, one red (outlining acinar cells) and one orange (outlining duct cells), that highlight a region of tissue that is shown at higher magnification on the right of each panel. For the immunostained images, different cell types are indicated as either a (acinar), d (intralobular duct), or sd (striated duct), and encircled regions of interest in either red (acinar) or orange (duct) show corresponding cells of interest. Transduced parotid glands show distinct hPTH staining, in a punctate appearance, throughout the gland (B). No polarity in hPTH expression was observed intracellularly and there were overwhelmingly more hPTH-positive acinar cells, compared with ductal cells. No significant signal was found in saline-infused animals (A). Representative pictures are shown.