Table 3. Determination of the association between metastasis/disease specific mortality and clinical parameters and HA-family members.

The pre- and post-operative parameters included age, gender, tumor grade, stage, lymph node status and concomitant presence of CIS.

Table 3A: Univariate analysis
Parameter	Chi-square	P-vale	Odds Ratio	Chi-square	P-Value	Odds Ratio
	Metastasis			Disease-specific mortality
Stage	8.64	0.003*	5.4; 2.2 – 22.4	8.6	0.0034	6.45; 2.4–29.4
Grade	0.01	0.94	ND	0.01	0.92	ND
Lymph node	4.53	0.033*	3.71; 1.3 – 16.4	5.82	0.016	3.9; 1.5–13.9
Gender	0.1	0.77	ND	0.00	1.0	ND
Age	0.13	0.73	ND	1.23	0.27	ND
CIS	0.74	0.39	ND	0.15	0.7	ND
HYAL-1	7.1	0.008*	1.76; 1.27 – 3.0	5.71	0.017	1.63; 1.2–2.7
HAS1	5.13	0.024*	1.83; 1.21 – 3.5	3.8	0.049	1.31; 1.1–2.0
HAS2	1.78	0.18	ND	0.02	0.9	ND
HAS3	2.86	0.09	ND	3.5	0.06	ND
RHAMM	0.46	0.5	ND	0	0.97	ND
CD44s	2.43	0.1	ND	0.77	0.38	ND
CD44v	0.7	0.4	ND	0.11	0.74	ND
CD44v/CD44s	0.81	0.37	ND	0.13	0.72	ND
HYAL-1 Staining*	10.9	0.009*	1.03; 1.02 – 1.06	4.13	0.04	1.36; 1.02 – 2.4
Table 3B: Multivariate analysis
Parameter	Chi-Square	P-value	Risk ratio; 95% CI	Chi-Square	P-value	Risk ratio; 95% CI
	Metastasis			Disease specific survival
Stage	5.66	0.0174*	5.7; 1.4–59.4	5.47	0.0193*	2.92; 1.2–8.3
Lymph node	7.26	0.007*	10.2; 1.9–71.4	1.9	0.17	ND
HYAL-1	5.35	0.019*	1.76; 1.1–2.85	3.98	0.046*	1.15; 1.05–1.28
HAS1	4.97	0.027*	1.37; 1.2–1.98	1.88	0.17	ND
HYAL-1-Staining	5.33	0.021*	1.65; 1.05–2.93	0.3	0.58	ND

A: Univariate analysis: Logistic regression analysis was used to determine the association of pre-and post-operative parameters and biomarker levels with outcome. ND: Not determined for parameters that did not reach significance.

^*: significant parameter.

B: Multivariate analysis: Cox proportional hazards analysis was performed by including all of the pre- and post-operative parameters and either the transcript levels or the staining scores of the HA-family molecules.