Fig. 3.

a Immunoblots for ANP, α-actin and calsequestrin in untreated cardiomyocytes and cardiomyocytes following 24 h treatment with ANG (1 μM) or PE (10 μM). Cardiomyocytes isolated from db/db animals (d) were also treated with the GFAT inhibitors azaserine (5 μM) or Don (20 μM). Mean densitometric data for b ANP and c α-actin expression normalized to calsequestrin from three individual experiments. Data presented as mean ± SE of five individual experiments. *P < 0.05 versus db/db group induced with ANG or PE