Fig. 2.

Kinetics of cold-shock induced apoptosis. Apoptosis induced by cold-shock in 32Dcl3 cells examined in flow cytometry by simultaneous staining for labeled annexin V (AnV) and propidium iodide (PI). a Prior to cold-shock most cells are not apoptotic. b 30 min after rewarming a high percentage of cells is early apoptotic. c After 90 min an even higher percentage of cells is early apoptotic. d After 5 h the percentage of late apoptotic cells increases e 24 h after cold-shock and rewarming most cells are late apoptotic. The average (±SD) of four experiments is depicted in panel f X-axis; hours of rewarming