Table 4.
Expression evidence of the transcripts and probes within Igf1
| Transcript or probe ID* |
Exon tagged* | Gene expression difference, MRL vs. SM†: fold change and (P value) |
QTL analysis‡ | ||
|---|---|---|---|---|---|
| Females | Males | LOD§ | High allele | ||
| Transcripts: | |||||
| ENSMUST00000075330 | 1b, 3, 4, 5a, 6d | +1.39 (0.002) | +1.19 (0.072) | 0.4 | - |
| ENSMUST00000095360 | 1b, 3, 4, 6d | +1.42 (0.001) | +1.16 (0.106) | 0.3 | - |
| ENSMUST00000062862 | 2a, 3, 4, 5b | +1.27 (0.024) | +1.10 (0.349) | 0.7 | - |
| Probes: | |||||
| 1054540 | 1b | - | - | 22.6 | SM |
| 317441 | 1b | - | - | 20.8 | SM |
| 732594 | 1b | - | - | 17.5 | SM |
| 817986 | 2 | - | - | 3.4 | SM |
| 533871 | 4 | - | - | 3.9 | MRL |
| 18947 | 6 | - | - | 3.2 | MRL |
| 231840 | 6d | - | - | 3.2 | MRL |
The probe IDs and the exons are referenced in Tables S4 and S5, Supporting Information.
Fold changes are represented as the mean in the MRL mice compared to the mean in the SM mice in males and females separately. P values were calculated by ANOVA analysis and are indicated in parentheses for the transcripts. No calculations were performed for the probes.
The microarray and QTL analysis was performed on 282 liver samples from the 371 F2 mice. The results of the QTL analysis in this sub-population was similar to what we found in the QTL analysis for the entire cohort. QTL analysis in the 282 F2 mice confirmed the Chr 10 QTL at 44 cM (LOD = 25.2) (Igf1q4), which explain about 30% of the IGF-1 variation.
LOD scores were calculated using the expression level as the phenotype. Sex was added as an additive covariate in the model. Max LOD scores are reported at the Igf1 locus.