Fig. 2.

Functional performance of universal RNA controls for real time qRT-PCR assays. Robust calibration control genes of MSG, CAB, RBS1, and ACTB at 0.1, 1, 10, and 1,000 pg over 38 individual 96-well reaction plates for Saccharomyces cerevisiae NRRL Y-50049 and NRRL Y-12632 treated with and without combined inhibitors of furfural and HMF at a final concentration of 20 mM each demonstrated highly fitted linear relationship between the mRNA input (log pg) and cycle numbers (Ct) by a master equation for assays on ABI 7500 real time PCR System. Standard deviation of the slope and the intercept of the master equation based on 38 individual standard curves under varied experimental conditions was 0.0549 and 0.1896, respectively