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Robust performance of standard control genes using CAB as sole reference to set a manual threshold at 26 Ct and a master equation derived from 38 replicated plate reactions on Applied Biosystems 7,500 real time PCR System
| Control gene | Reference Ct | Mean Ct | Stdev | Estimated mRNA (pg) | Input mRNA (pg) | Amplification efficiency |
|---|---|---|---|---|---|---|
| MSG | 29.349 | 0.189 | 0.105 | 0.1 | 0.95 | |
| CAB | 26.0 | 25.942 | 0.18 | 0.939 | 1 | 0.94 |
| RBS1 | 22.267 | 0.111 | 9.97 | 10 | 1.00 | |
| ACTB | 15.072 | 0.027 | 1017.448 | 1000 | 0.98 |
