Fig. 1.
T3 regulated cell multiplication and protein synthesis of MC3T3-E1 osteoblastic cells. Cells were cultured in absence (Co) or presence of T3 (10−7 M) for the indicated time. During culture T3 attenuated cell multiplication beginning with day 4 (A) significantly (P ⩽ 0.001). The effect of T3 was dose dependent. T3 significantly increased cell multiplication at 10−9 and 10−8 M on day 4 (B) while on day 12 (C) T3 attenuated cell multiplication at 10−8, 10−7 and 10−6 M, significantly. On day 12, T3 increased protein synthesis significantly when normalized to the DNA-amount. The bars indicate mean ± SD. ∗P ⩽ 0.05; ∗∗P ⩽ 0.01; ∗∗∗P ⩽ 0.001; Co vs. treatments (n = 4).