Table 1.
Bacterial strains and plasmids.
| Strain/plasmid | Relevant characteristics or use | Source/reference |
|---|---|---|
| Bacillus subtilis subsp. subtilis str. 168 | Multiple sequence alignment | NC_000964 |
| Bacteroides fragilis NCTC 9343 | Multiple Sequence alignment | NC_003228 |
| Bacteroides thetaiotoamicron VP1-5482 | Multiple sequence alignment | NC_004663 |
| Deinococcus radiodurans R1 | Multiple sequence alignment | NC_001263 |
| Escherichia coli K-12 | Multiple sequence alignment | NC_000913 |
| Porphyromonas gingivalis W83 | Multiple sequence alignment | NC_002950 |
| Bacteroides fragilis 638R | Multiple sequence alignment | Privitera et al., 1979 |
| Bacteroides fragilis | ||
| 638R | Clinical strain, RifRGentR | Privitera et al., 1979 |
| 638R recA | 638R derivative, recA RifRGentRErmR | This study |
| 638R (pLYL01) | 638R RifRGentRTetR | This study |
| 638R recA (pLYL01) | 638R recA RifRGentRErmRTetR | This study |
| 638R recA(pLYLrecA) | 638R recA (recA+) RifRGentRErmRTetR | This study |
| 638R (pLYLrecA) | 638R (recA+) RifR GentR TetR | This study |
| Escherichia coli | ||
| S17-1 | RP4-2-Tc::Mu aph::Tn7 recA StrepR | Simon et al., 1983 |
| S17-1 (pGREC) | S17-1 containing pGREC | This study |
| S17-1 (pLYL01) | S17-1 containing pLYL01 | This study |
| S17-1 (pLYLrecA) | S17-1 containing pLYLrecA | This study |
| Plasmids | ||
| pGERM | pUC19-based suicide vector, ErmR | Shoemaker et al., 2000 |
| pGREC | pGERM containing recA internal fragment | This study |
| pLYL01 | Mob+, TetRAmpR | Li et al., 1995 |
| pLYLrecA | pLYL01 containing B. fragilis recA | This study |
Rif, rifampicin; Gent, gentamicin; Erm, erythromycin; Tet, tetracycline; Strep, streptomycin; Amp, ampicillin; Mob, mobilisation; R, resistant; S, sensitive.