Table 3.
Primers used.
| Name | Primer | Description | Reference |
|---|---|---|---|
| FRA | 5′-GTA AAG CTG CAG ATG AAG TGA TCG-3′ (PstI) | FRA and RRA amplify the full-length B. fragilis recA BF638R1245 gene. Restriction enzyme sites (in brackets) are underlined. | This study |
| RRA | 5′-GGG CAT GCC TAT CGA GTT GG-3′ (SphI) | This study | |
| FBRT | 5′-CCG GCT ATG ATC GGT GCC-3′ | FBRT and RBRT amplify the intergenic region between BF638R1248 and BF638R1246/7. | This study |
| RBRT | 5′-CGG CTT TAC GTA GCT CTG CG-3′ | This study | |
| RART | 5′-CGT GGA TGG CCA GTG TCG-3′ | FRA and RART amplify the intergenic region between BF638R1246/7 and BF638R1245. | This study |
| M13F | 5′-CGC CAG GGT TTT CCC AGT CAC GAC-3′ | M13F and M13R in combination with gene-specific primers allow verification of mutation in B. fragilis 638R recA. | Val Abratt Yanisch-Perron et al., 1985 |
| M13R | 5′-GAG CGG ATA ACA ATT TCA CAC AGG-3′ | Val Abratt Yanisch-Perron et al., 1985 | |
| RIF | 5′-CAG GTT CGA TAG CAC TGA ATG C-3′ | RIF and RIR amplify an internal fragment of the B. fragilis recA BF638R1245 gene; used for the mutation of recA | This study |
| RIR | 5′-CGG ATT ACC GAA CAT TAC ACC G-3′ | This study |