Fig. 1.

Sequence of C-terminal human NM-MHC IIA fragments and peptides. The His-tagged expressed fragments M200 and M111 contained an N-terminal fusion comprising residues MRGSHHHHHGS. The sequence shown corresponds to M200 (residues Q1761-E1960; native C-terminus). M111 starts at residue Q1850 (red). The M32 peptide (A1907-G1938) is underlined, M16^N (D1908-R1923) is shown in green, and M15^C (G1924-G1938) is shown in blue. Residues in the a and d heptad repeat positions of the coiled coil (generally hydrophobic) are shown in boldface. The nonhelical C-terminus region is shown in italics. Note that the numbering system is one residue shorter than that originally used by Kriajevska et al. and corresponds to the current nonmuscle myosin IIA database nomenclature (NCBI reference sequence NM_002473.4).⁹ Thus, M200 is equivalent to the Hmyo4-3B fragment.⁹ Note that the M200 and M111 preparations contain a V1913I substitution.