Figure 7.

Effects of DNE3 on the activation of NF-κB, c-Jun, and c-Fos. Cells were treated with DNE3 and then nuclear extracts were analysed for DNA binding activity of NF-κB (a) and AP-1 (b) using biotin labeled NF-κB and AP-1 specific oligonucleotide in EMSA. The last lane represented nuclear extracts incubated with unlabeled oligonucleotide (comp) to confirm the specificity of binding. Nuclear and cytoplasmic extracts were subjected to SDS-PAGE followed by western blotting with anti-NF-κB, c-Fos, c-Jun, or C23 antibodies (c) and anti-IkBα or β-actin antibodies, respectively. Signals of proteins were visualized with an ECL detection system. The experiments were repeated three times with similar results.