Fig. 1.

Tamoxifen-induced deletion of Tln1 in mouse embryos results in widespread bleeding.

(A) 10.5 dpc Tln1^fl/fl;CreER embryo possessing the ROSA26 lacZ reporter exposed to tamoxifen in utero 48 h earlier (left panel) followed by staining with X-gal (right panel). (B) Mouse embryo fibroblasts of the genotypes shown were treated with 4OHT, and 96 h later cell lysates were blotted and probed with talin1 antibody 97 H6. Vinculin was used as a loading control. (C) Examples of 10.5 dpc embryos of genotypes shown exposed to tamoxifen in utero 48 h earlier at 8.5 dpc. Arrows indicate site of abnormal blood accumulation. (D) Examples of 11.5 dpc embryos of genotypes shown exposed to tamoxifen in utero 72 h earlier at 8.5 dpc. (E) 9.0 dpc embryos of genotypes shown exposed to tamoxifen in utero 48 h earlier at ~ 7.0 dpc. Bars = 0.5 cm. (F) 12.5 dpc embryos of genotypes shown exposed to tamoxifen in utero 48 h earlier. Boxed regions in (a) and (b) are magnified in (c) and (d), respectively. H&E stained sections of the embryo in (b) showing sites of bleeding (arrows) in ganglion VIII (e) and lung primordia (f). Bars = 0.5 cm (a–d) and 100 μm (e, f).