Fig. 2.

Tln1 CreER embryos show defective blood vessel organisation.

(A) 10.5 dpc embryos of genotypes shown were exposed in utero to tamoxifen at ~ 8.5 dpc and were then sectioned and stained with antibody to the endothelial-specific marker, endomucin (brown). Sections shown are of the lateral neural tube at the cervical region. The mutant vessels are often not completely enclosed (arrow) and packed with blood cells (*). In some areas blood cells can been seen outside the vessels (arrowhead). Bars = 50 μm. (B) 10.5 dpc Tln1^fl/fl;CreER (mut) and Tln1^fl/+;CreER (con) embryos exposed to tamoxifen in utero 48 h earlier were whole mount stained for PECAM1, then imaged on a fluorescent stereomicroscope; only boxed regions shown in left panels are shown (a–d). The mutant vessels (b, d) are dilated (open arrowhead), discontinuous (arrow) and endothelial cells are not organised into vessels (arrowhead). Bars = 100 μm.