Fig. 4.

Talin1 is the only isoform expressed in endothelial cells and as such is necessary for cell spreading in vitro.

(A) Western blots of lysates from two separate MLEC lines and embryonic kidney probed with talin1 (T1) and talin2 (T2) specific antibodies. Vinculin (Vinc) was used as a loading control. (B) Primary embryonic endothelial cells cultured on coverslips stained with antibodies to either talin1 (T1) or talin2 (T2) (green) along with antibodies to PECAM1 (red) and actin (blue). Talin2 was detected only in the PECAM negative non-endothelial cells (inset). (C) MLEC from Tln1^fl/fl;CreER mice were treated with either 100 nM 4OHT or ethanol and stained 72 h later for talin1 (green). Bars = 20 μm.