Fig. 5.

Tln1 null endothelial cells are unable to spread and flatten in vivo.

(A) Confocal maximum intensity projections of 9.5 dpc Tie2-Cre embryos whole mount stained for PECAM1 (genotypes shown). Higher power views of the boxed regions in (a) and (d) are shown in (b) and (c) and (e) and (f), respectively; Bars = 200 μm (a, d) and 20 μm (b, c, e, f). (B) Electron micrographs of ultrathin sections through the heads of X-gal stained 9.5 dpc embryos of genotypes shown. The endothelial cells (ec) and in some cases the blood cells (bc; which can also express Tie2-Cre) are identifiable due to the insoluble electron dense deposits of bromo-chloro-indolyl-galactoside product that appear as black spots within the cells. Bar = 5 μm.