Figure S1.
(A) 11.5 dpc embryos of genotypes shown, exposed to tamoxifen in utero 48 h earlier at ~ 9.5 dpc. (B) Western blot of protein lysates from Tln1fl/fl;CreER fibroblasts 24 or 96 h after they were treated with 4OHT (+) or ethanol (−). Cells at 96 h were also trypsinised, replated and lysates made 24 h after replating (96R). Blots were probed with 97 H6 talin1-specific antibody, with an anti-vinculin (Vinc) antibody serving as a loading control. (C) Morphology of Tln1fl/−;CreER fibroblasts treated either with 4OHT or ethanol vehicle. Cells were photographed at 24 and 96 h after addition of 4OHT/ethanol. Alternatively, cells were trypsinised at 96 h, replated and photographed a further 24 h later (96R). Arrows indicate cells that have failed to remain spread.