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. Author manuscript; available in PMC: 2011 Jan 24.
Published in final edited form as: Free Radic Res. 2010 Mar;44(3):313–321. doi: 10.3109/10715760903494168

Figure 1.

Figure 1

Effect of high glucose on mitochondrial DNA damage: DNA damage was assessed using 15 ng total DNA and mitochondrial or nuclear specific primers for long and short PCR products in the cells incubated with 5 mM glucose or 20 mM glucose in the presence of MnTBAP or from the cells transfected with MnSOD or Mock cells incubated in 5 mM or 20 mM glucose media. (A) Relative amplification was calculated by normalizing the intensity of the 13.3 kb product to the 232 bp product for mtDNA and (B) the 13.8 kb product to the 241 bp product for nDNA. Each measurement was made in duplicate in at least three different cell preparations. 5=5 mM glucose, 20=20 mM glucose, 20+MnTb=20 mM glucose + 200 μM MnTBAP, SOD=cells transfected with MnSOD, Mock=cells treated with the transfection reagent alone. *p < 0.05 compared to the values obtained from the un-transfected cells incubated in 5 mM glucose.