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. Author manuscript; available in PMC: 2011 Jan 24.
Published in final edited form as: Free Radic Res. 2010 Mar;44(3):313–321. doi: 10.3109/10715760903494168

Figure 3.

Figure 3

Effect of MnSOD on the co-localization of superoxide radicals and OGG1 protein: at the end of incubation in high glucose (4 days) the cells were incubated with Mitotracker Red, washed and fixed with formaldehyde. The cells were permeabilized with Triton X and immunostained for OGG1 using FITC-conjugated secondary antibody. The picture is representative of five different experiments. The accompanying histogram represents the intensity of Mitotracker Red quantified by MetaMorph software in a constant area and constant threshold. 5 mM glucose and 20 mM glucose represent un-transfected cells incubated in 5 mM or 20 mM glucose and 20 mM glucose+SOD=MnSOD transfected cells incubated with 20 mM glucose for 4 days.