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Effect of MnSOD on mitochondrial GSH and membrane permeability: mitochondria were prepared from the cells transfected with MnSOD or untransfected, incubated in the presence or absence of MnTBAP. (A) GSH levels were quantified by a colourimetric method and (B) membrane permeability by a spectrophotometric method by measuring the decrease in absorbance at 540 nm induced by calcium chloride.
