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. Author manuscript; available in PMC: 2011 Jan 24.
Published in final edited form as: Free Radic Res. 2010 Mar;44(3):313–321. doi: 10.3109/10715760903494168

Table I.

Bovine specific primers for detection of DNA damage and mitochondrial-encoded genes.

Target Primer sequence (5′ to 3′) Product (base pairs)
mtDNA-long 13 353
 forward ATG AGT TGG TAG TTT CGG TTG GGG TG
 reverse ATT CTG TGG TCT GTG TAT GGG CGT GT
mtDNA-short 232
 forward CAT ACT CCT CTG TAA GCC ACA TAG C
 reverse AGA CTT GCT AGT AGT CAT CAG GTG G
nDNA-long 13 823
 forward ACA TGC TCC AAC GGC TGC AA
 reverse TGG CAG GGT CCG AAA ATT GCT
nDNA-short 241
 forward TAA GGC TCC CTG CCT CTA CA
 reverse ACC CTT CAA CTT CCA CGA TG
ND1 297
 forward AGG ACC ATT TGC CCT CTT CT
 reverse GGT GGG ATG CCT GAT GTA AG
ND4 269
 forward CCT ACA AAC GCT CCT TCC AC
 reverse TGA GTG CAT TTT CCC GTG TA
ND6 250
 forward CGT GAT AGG TTT TGT GGG GT
 reverse GCC AGT AAC AAA TGC CCC TA
Cytochrome b 298
 forward CGA TAC ATA CAC GCA AAC GG
 reverse AGA ATC GGG TAA GGG TTG CT
β-actin 205
 forward CCTCTATGCCAACACAGTGC
 reverse CATCGTACTCCTGCTTGCTG