Fig. 5.
Cathepsin B siRNA effectively inhibited cathepsin B in HepG2 cells. Cathepsin B expression in HepG2 cells was silenced by siRNA as detailed in the Materials and Methods section. After transfection of HepG2 cells with cathepsin B siRNA, (A) an immunoblot for Bax and β-actin was performed, and (B) cathepsin B activity was measured using a cathepsin B activity assay as detailed in the Materials and Methods section (*P < 0.01 compared with control; Ctsb, cathepsin B; Scr., scramble; siRNA, small interfering RNA.