Figure 4.
Sequential images of yeast cell when passing through the intersection. (a) Small (white arrows, 1 and 2) and large (yellow arrows, 3, 4, and 5) yeast cells initially flowing in the bottom channel. (b) When they encounter the line laser (dashed box), the large yeast cell was stopped and pushed to upper channel while the small yeast cell passed straight through. (c) and (d) The cells that are moving to the top channel become out of focus and disappear from the image when it is totally switched to top channel. The large cell and small cell are successfully separated and are readily collected at two different outlets. The sorting box was 25×25×80 μm3 in size. The cells were moving at speeds of ∼30 μm∕s. The blue arrows show the direction of fluid flow (enhanced online). 1