Figure 1.

Induced expression of wild-type and mutant TDP-43 in HEK293 cells. Tagged forms of TDP-43 (F-TDP43) were expressed for 24 or 72 h on tetracycline (Tet) induction (1 μg/ml). The diagrams on the left are a schematic representation of the different TDP-43 protein domains, numbering denotes amino acid residues. The N-terminal domain tag is represented by the hashed box and the RNA recognition motifs 1 and 2 are shown as black and grey boxes, respectively. Deletion of the C-terminal region in mutant Δ321–366 is depicted as a white box. The right panels are immunoblot analyses of the protein extracts with an antibody against TDP-43 that detects both endogenous (end) and tagged (TG-TDP43) protein. Tubulin was used as loading control.