Figure 2.

TDP-43 negative autogenous regulation occurs at the transcript level. (A) Schematic representation of the TDP-43 gene with coding exons and untranslated regions shown in black and grey, respectively. The various predicted polyadenylation signals are indicated, where (^*) denotes the experimentally validated sites. The two principally expressed endogenous isoforms (V1pA₁ and V1pA₄) are shown including the transgenic TG-TDP43 transcript containing the coding sequences only. The V2 form corresponds to a minor isoform discussed in Supplementary Figure 2. (B) Northern blot analysis of control and after 24 and 72 h of induction. V1pA₁ and V1pA₄ correspond to the 2.8 and 4.2 Kb bands, respectively. Endogenous TDP-43 transcripts were detected by probing the region indicated by the arrows in (A), while TG-TDP43 was identified with a probe spanning exons 2 and 3. GAPDH detection was used as loading control.