Figure 5.

TDP-43 autoregulation is independent of NMD. (A) The existence of alternative splicing that activates the removal of two downstream introns (V2) was seen in the EST database. The shorter form V2 was seen to undergo NMD (Supplementary Figure 2), while northern blot analysis (B) of control and Tet-induced samples in the presence and absence of CHX treatment (50 μg/ml, for 3 h) did not detect the presence of V2. V2 should have a MW of 1.2 or 2.5 Kb depending on the poly A site usage. The probe was generated with the primers shown in (A) followed by purification of the product corresponding to V2.